|
KMID : 0604520140400040403
|
|
Journal of the Society of Cosmetic Scientists of Korea
2014 Volume.40 No. 4 p.403 ~ p.412
|
|
|
Inhibition of Melanogenesis by Cucurbitacin B from Cucumis sativus L.
|
|
Chang Yun-Hee
Choo Jung-Ha
Lee So-Young
Kim Tae-Yoon
Jin Mu-Hyun
Chang Min-Youl
Lee Sang-Hwa
Lee Cheon-Koo
Park Sun-Gyoo
|
|
|
Abstract
|
|
|
|
To develop an effective skin whitening agent for cosmetics, we isolated cucurbitacin B from Cucumis sativus L. which has been used as traditional skin lighting regimen by the bioactivity-guided fractionation, and investigated the inhibitory effects of cucurbitacin B on melanogenesis. At a non-cytotoxic concentration, cucurbitacin B reduced melanin contents of B16F1 melanoma cells in a dose-dependent manner. Cucurbitacin B did not directly inhibit mushroom tyrosinase activity, but it inhibited intracellular tyrosinase activity in a dose-dependent manner. Its inhibitory mechanism on melanin biosynthesis was further assessed, and we found that cucurbitacin B significantly decreased the protein level of tyrosinase, a major melanogenic enzymes and MITF, a master transcriptional factor of melanogenesis. In addition, cucurbitacin B increased the expression of WW domain-containing oxidoreductase (WWOX) which is known to function as tumor repressor and inhibits Wnt/β-catenin pathway. Collectively, these results suggest that cucuritacin B from C. sativus could be used as an active ingredient for skin whitening.
|
|
|
KEYWORD
|
|
|
cucurbitacin B, melanogenesis, tyrosinase, mitf, wwox
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|